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Evaluation of the Influence of the Conjugation Site of the Chelator Agent HYNIC to GLP1 Antagonist Radiotracer for Insulinoma Diagnosis

[ Vol. 10 , Issue. 1 ]

Author(s):

Bluma Linkowski Faintuch, Daniele Seo, Erica Aparecida de Oliveira, Roselaine Campos Targino and Ana Maria Moro   Pages 65 - 72 ( 8 )

Abstract:


Background and Objective: Radiotracer diagnosis of insulinoma, can be done using somatostatin or glucagon-like peptide 1 (GLP-1). Performance of GLP-1 antagonists tends to be better than of agonists.

Methods: We investigated the uptake of the antagonist exendin (9-39), radiolabeled with technetium- 99m. Two different sites of the biomolecule were selected for chelator attachment.

Results: HYNIC-βAla chelator attached to serine (C- terminus) of exendin, was associated with higher tumor uptake than to aspartate (N- terminus).

Conclusion: The chelator position in the biomolecule influenced receptor uptake.

Keywords:

Insulinoma, GLP-1, antagonist, exendin (9-39), radiotracer, Technetium-99m.

Affiliation:

Institute of Energy and Nuclear Research Radiopharmacy Center Av. Prof. Lineu Prestes, 2242 São Paulo, Radiopharmacy Center, Institute of Energy and Nuclear Research, São Paulo, Radiopharmacy Center, Institute of Energy and Nuclear Research, São Paulo, Laboratory of Biopharmacology in Animal Cells, Butantan Institute, Sao Paulo, Laboratory of Biopharmacology in Animal Cells, Butantan Institute, Sao Paulo

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